The biochemical characterization of sugar uptake in yeasts started five decades ago and led
to the early production of abundant kinetic and mechanistic data. However, the first accurate
overview of the underlying sugar transporter genes was obtained relatively late, due mainly …

Transport of glucose and fructose in the yeast Saccharomyces cerevisiae plays a crucial role
in controlling the rate of wine fermentation. In S. cerevisiae, hexoses are transported by
facilitated diffusion via hexose carriers (Hxt), which prefer glucose to fructose. However …

The major facilitator superfamily (MFS) is one of the two largest superfamilies of membrane
transporters present ubiquitously in bacteria, archaea, and eukarya and includes members
that function as uniporters, symporters or antiporters. We report here the complete …

Abstract d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which
expressed the gene for NADP+-dependent d-xylose dehydrogenase from Trichoderma
reesei (xyd1). Up to 19±2 g d-xylonate l− 1 was produced when K. lactis expressing xyd1 …

The use of more concentrated, so-called high-gravity and very-high-gravity (VHG) brewer's
worts for the manufacture of beer has economic and environmental advantages. However,
many current strains of brewer's yeasts ferment VHG worts slowly and incompletely, leaving …

Candida albicans accumulates large amounts of the polyols glycerol and d-arabitol when
the cells are exposed to physiological conditions relevant to stress and virulence in animals.
Intracellular concentrations of glycerol are determined by rates of glycerol production and …

As one of the best xylose utilization microorganisms, Scheffersomyces stipitis exhibits great
potential for the efficient lignocellulosic biomass fermentation. Therefore, a comprehensive
understanding of its unique physiological and metabolic characteristics is required to further …

Acetic acid is present in cellulosic hydrolysate as a potent inhibitor, and the superior acetic
acid tolerance of Saccharomyces cerevisiae ensures good cell viability and efficient ethanol
production when cellulosic raw materials are used as substrates. In this study, a mutant …

Lactose is intracellularly hydrolysed by Aspergillus nidulans. Classical mutation mapping
data and the physical characteristics of the previously purified glycosyl hydrolase facilitated
identification of the clustered, divergently transcribed intracellular β-galactosidase (bgaD) …

As genome sequencing outstrips the rate of high-quality, low-throughput biochemical and
genetic experimentation, accurate annotation of protein function becomes a bottleneck in the
progress of the biomolecular sciences. Most gene products are now annotated by homology …